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Reading: Micronucleus assay as a triage tool for borderline cases of cervical dysplasia

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Research Article

Micronucleus assay as a triage tool for borderline cases of cervical dysplasia

Authors:

Deeksha Pandey ,

Kasturba Medical College, IN
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Sahitya Putteddy,

Kasturba Medical College, IN
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Satish Rao

Manipal Life Science Centre, Manipal University, IN
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Abstract

Objective: In cases of cervical carcinogenesis the gradient from low to high-grade lesions is characterized by increasing nuclear atypia and the failure of cellular differentiation. These phenotypic changes are presumed to be accompanied by increased genetic instability that can be documented using the micronucleus (MN) assay in exfoliated cervical cells.

Material and methods: This blinded prospective study included 169 samples for final analysis; 119 normal cervical cytology, 18 preinvasive lesions and 33 frank carcinoma. A thin smear was prepared from each sample on a glass slide, which was then stained with acridine orange (a DNA specific dye). Around thousand cells per slide were counted under fluorescent microscope, 20-40 X magnification. Number of micro-nucleated cells and total number of micronuclei were recorded to calculate MN cell ratio and total MN ratio.

Results: Micro-nucleated cells as well as the total number of micronuclei show increasing trend towards malignization. By ROC curve analysis, for MN cell ratio of 0.1999, the sensitivity is found to be as high as 91.4% with a specificity of 82.8%. Similar results were obtained when total MN ratios were analyzed. Advantage of combining both the ratios together does not confer any additional benefit in terms of sensitivity and specificity.

Key words: micro-nucleus (MN); cervical cancer; lowgrade squamous intraepithelial lesion (LSIL); highgrade squamous intraepithelial lesion (HSIL).

DOI: http://dx.doi.org/10.4038/sljog.v33i3.4018

Sri Lanka Journal of Obstetrics and Gynaecology 2011; 33: 104-111

How to Cite: Pandey, D., Putteddy, S. & Rao, S., (2012). Micronucleus assay as a triage tool for borderline cases of cervical dysplasia. Sri Lanka Journal of Obstetrics and Gynaecology. 33(3), pp.104–111. DOI: http://doi.org/10.4038/sljog.v33i3.4018
Published on 23 Jan 2012.
Peer Reviewed

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